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ContactsFormats of diagnostic kits
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Lyophilized ready-to-use reaction mixture in white strips in a 96-well plate (8 x 0.2 ml tubes) to which isolated DNA/RNA samples are added.
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Veterinary diagnostics
Veterinary diagnostics of DNA/RNA causative agents of infectious diseases using the Real-Time PCR method.
Veterinary diagnostic kits RealBest-Vet® are designed for the detection of nucleic acids of microbiological agents isolated from clinical samples of animals, causing various infectious diseases. Detection is achieved using the Real-Time PCR method with hybridization-fluorescence TaqMan probes.
- Comprehensive approach to PCR diagnostics includes sample preparation (DNA/RNA extraction), Real-Time PCR, and result evaluation.
- Simple extraction of nucleic acids using magnetic particles.
- Uniform PCR and RT-PCR protocols for the detection of a wide range of pathogens.
- Non-competitive internal control.
- Storage of all kit components at the same temperature of +2 to +8 °C for 12 months.
- Transport at a temperature of up to +25 °C for a maximum of 10 days.
- Compliance with the requirements of EN ISO 13485:2016.
- All necessary components for the PCR reaction are part of the lyophilized reaction mixture; in the case of reverse transcription PCR (RT-PCR), a separate reverse transcription step is not required.
- To set up the PCR reaction, simply add extracted DNA/RNA samples and run PCR or RT-PCR.
Real-Time PCR devices and formats
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CFX96 (Bio-Rad, USA), DTlite, DT-96 (DNA-Technology, Russia)
Number of simultaneously detectable pathogens |
Detection channels |
|---|---|
| 1 | FAM, ROX |
| 2 | FAM, ROX, HEX (JOE) |
| 3 and more | FAM, ROX, HEX (JOE), Cy5 |
- Other Real-Time PCR instruments with similar technical characteristics can be used after validation.
Zloženie diagnostických súprav
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Reaction mixture - lyophilized, ready for use
Positive control sample
96/48 white 0.2 ml microtubes (8 strips of 12 microtubes each) in a PCR plate
1 tube, 1 ml