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ContactsFormats of diagnostic kits
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lyophilized ready-made reaction mixture in white strips to which the isolated DNA sample is added
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Genetics
Diagnosis of Genetic Polymorphisms Using the Real-Time PCR Method.
Genetic kits RealBest® are designed for the determination of genetic polymorphisms from clinical samples causing various genetic diseases. The detection is performed using the Real-Time PCR method with melting curve analysis.
The company Diagnostické centrum DNK, s.r.o. is a distributor of diagnostic kits RealBest®.
- comprehensive approach to PCR diagnostics includes extraction of human DNA, Real-Time PCR and evaluation of results
- easy DNA extraction using magnetic particles
- lyophilized reaction mixtures for amplification of isolated DNA are ready for use
- Uniform PCR protocols for the detection of a wide range of genetic polymorphisms
- storage of all kit components at the same temperature +2 to +8 °C for 12 months
- transport at a temperature up to +25 °c max. 10 days
- compliance with the requirements of EN ISO 13485:2016
- all necessary components for the PCR reaction are part of the lyophilized reaction mixture
- to set up the PCR reaction, just add the extracted DNA samples and start the PCR
Real-Time PCR devices and formats
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CFX96 (Bio-Rad, USA) for all kits
DT-96 (DNA-Technology, Russia) only for kits 3807, 3808, 3809, 3810, 3814, 3816, 3822, 3823, 3824, 3836, 3841
Number of simultaneously detectable polymorphisms |
Detection channels |
|---|---|
| 1 | FAM, ROX |
| 2 | FAM, ROX, HEX (JOE) |
| 3 and more | FAM, ROX, HEX (JOE), Cy5 |
- Other Real-Time PCR instruments with similar technical characteristics can be used after validation.
The composition of diagnostic kits
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Reaction mixture – lyophilized, ready for use
Positive control sample
48 white 0.2 ml microtubes in a PCR plate (in strips)
1 tube, 1 ml