Real-Time PCR Diagnostic Kits - Banner

Diagnosis of Genetic Polymorphisms Using the Real-Time PCR Method.

Genetic kits RealBest® are designed for the determination of genetic polymorphisms from clinical samples causing various genetic diseases. The detection is performed using the Real-Time PCR method with melting curve analysis.

The company Diagnostické centrum DNK, s.r.o. is a distributor of diagnostic kits RealBest®.

  • comprehensive approach to PCR diagnostics includes extraction of human DNA, Real-Time PCR and evaluation of results
  • easy DNA extraction using magnetic particles
  • lyophilized reaction mixtures for amplification of isolated DNA are ready for use
  • Uniform PCR protocols for the detection of a wide range of genetic polymorphisms
  • storage of all kit components at the same temperature +2 to +8 °C for 12 months
  • transport at a temperature up to +25 °c max. 10 days
  • compliance with the requirements of EN ISO 13485:2016

Formats of diagnostic kits


lyophilized ready-made reaction mixture in white strips to which the isolated DNA sample is added

Illustrative image
  • all necessary components for the PCR reaction are part of the lyophilized reaction mixture
  • to set up the PCR reaction, just add the extracted DNA samples and start the PCR

Real-Time PCR devices and formats


CFX96 (Bio-Rad, USA) for all kits

DT-96 (DNA-Technology, Russia) only for kits 3807, 3808, 3809, 3810, 3814, 3816, 3822, 3823, 3824, 3836, 3841

Illustrative image

Number of simultaneously detectable polymorphisms

Detection channels

3 and more FAM, ROX, HEX (JOE), Cy5
  • Other Real-Time PCR instruments with similar technical characteristics can be used after validation.

The composition of diagnostic kits


Reaction mixture – lyophilized, ready for use
Positive control sample

48 white 0.2 ml microtubes in a PCR plate (in strips)
1 tube, 1 ml

Diagnostic kits (PDF)