Microbiology

Real-Time PCR Diagnostic Kits - Banner

Diagnostics of DNA/RNA causative agents of infectious diseases using the Real-Time PCR method.

Diagnostic kits RealBest® are designed for the detection of nucleic acids of microbiological agents isolated from clinical samples, causing various infectious diseases. The detection is carried out using the Real-Time PCR method with hybridization-fluorescence TaqMan probes. Techniques developed by the manufacturer allow minimizing the number of required steps and guaranteeing maximum result stability. All diagnostic kits RealBest® are presented in the form of lyophilized reaction mixtures and are adapted to work on the most commonly used Real-Time instruments of the plate and rotor types.



The company Diagnostické centrum DNK, s.r.o. is a distributor of diagnostic kits RealBest®.



  • Comprehensive approach to PCR diagnostics includes sample preparation (DNA/RNA extraction), Real-Time PCR, and result evaluation.
  • Simple DNA extraction using magnetic particles.
  • Lyophilized reaction mixtures for amplification of isolated DNA/RNA are ready for use.
  • Uniform PCR and RT-PCR protocols for the detection of a wide range of pathogens.
  • Non-competitive internal control.
  • Storage of all kit components at the same temperature of +2 to +8 °C for 12 months.
  • Transport at a temperature of up to +25 °C for a maximum of 10 days.
  • Compliance with the requirements of EN ISO 13485:2016.

Formats of diagnostic kits

Str

Lyophilized ready-to-use reaction mixture in white strips in a 96-well plate (8 x 0.2 ml tubes), to which isolated DNA/RNA samples are added.

Uni

Lyophilized ready-to-use reaction mixture in clear 0.2 ml tubes, to which isolated DNA/RNA is added, one tube equals one reaction.

Illustrative image
  • All necessary components for the PCR reaction are part of the lyophilized reaction mixture. In the case of PCR with reverse transcription (RT-PCR), a separate reverse transcription step is not required.
  • To set up the PCR reaction, it is sufficient to add samples of extracted DNA/RNA and initiate PCR or RT-PCR.

Real-Time PCR devices and formats

Str

CFX96 (Bio-Rad, USA), DTprime (DNA-Technology, Russia),
FLUORITE® (Tianlong Science&Technology, China).

Uni

CFX96 (Bio-Rad, USA), DTprime (DNA-Technology, Russia),
Rotor-Gene Q (Qiagen, Germany),
Rotor-Gene 3000/6000 (Corbett Research, Australia),
FLUORITE® (Tianlong Science&Technology, China).

Illustrative image

Number of simultaneously detectable pathogens

Detection channels
1 FAM, ROX
2 FAM, ROX, HEX (JOE)
3 and more FAM, ROX, HEX (JOE), Cy5
  • Other Real-Time PCR instruments with similar technical characteristics can be used after validation.

The composition of diagnostic kits

Str

Reaction mixture – lyophilized, ready for use
Positive control sample

96/48 white 0.2 ml microtubes (8 strips of 12 microtubes each) in a PCR plate, 1 tube, 1 ml

Uni

Reaction mixture – lyophilized, ready for use
Positive control sample

96/48 separate colorless 0.2 ml microtubes in a PCR plate, 1 tube, 1 ml

Diagnostic kits (PDF)